OsUGE2 Regulates Plant Growth through Affecting ROS Homeostasis and Iron Level in Rice.

BACKGROUND: The growth and development of rice (Oryza sativa L.) are affected by multiple factors, such as ROS homeostasis and utilization of iron. Here, we demonstrate that OsUGE2, a gene encoding a UDP-glucose 4-epimerase, controls growth and development by regulating reactive oxygen species (ROS) and iron (Fe) level in rice. Knockout of this gene resulted in impaired growth, such as dwarf phenotype, weakened root growth and pale yellow leaves. Biochemical analysis showed that loss of function of OsUGE2 significantly altered the proportion and content of UDP-Glucose (UDP-Glc) and UDP-Galactose (UDP-Gal). Cellular observation indicates that the impaired growth may result from decreased cell length. More importantly, RNA-sequencing analysis showed that knockout of OsUGE2 significantly influenced the expression of genes related to oxidoreductase process and iron ion homeostasis. Consistently, the content of ROS and Fe are significantly decreased in OsUGE2 knockout mutant. Furthermore, knockout mutants of OsUGE2 are insensitive to both Fe deficiency and hydrogen peroxide (H2O2) treatment, which further confirmed that OsUGE2 control rice growth possibly through Fe and H2O2 signal. Collectively, these results reveal a new pathway that OsUGE2 could affect growth and development via influencing ROS homeostasis and Fe level in rice.

Micro/nanoplastics: Critical review of their impacts on plants, interactions with other contaminants (antibiotics, heavy metals, and polycyclic aromatic hydrocarbons), and management strategies.

Microplastic/nanoplastics (MPs/NPs) contamination is not only emerging threat to the agricultural system but also constitute global hazard to the environment worldwide. Recent review articles have addressed the environmental distribution of MPs/NPs and their single-exposure phytotoxicity in various plant species. However, the mechanisms of MPs/NPs-induced phytotoxicity in conjunction with that of other contaminants remain unknown, and there is a need for strategies to ameliorate such phytotoxicity. To address this, we comprehensively review the sources of MPs/NPs, their uptake by and effects on various plant species, and their phytotoxicity in conjunction with antibiotics, heavy metals, polycyclic aromatic hydrocarbons (PAHs), and other toxicants. We examine mechanisms to ameliorate MP/NP-induced phytotoxicity, including the use of phytohormones, biochar, and other plant-growth regulators. We discuss the effects of MPs/NPs -induced phytotoxicity in terms of its ability to inhibit plant growth and photosynthesis, disrupt nutrient metabolism, inhibit seed germination, promote oxidative stress, alter the antioxidant defense system, and induce genotoxicity. This review summarizes the novel strategies for mitigating MPs/NPs phytotoxicity, presents recent advances, and highlights research gaps, providing a foundation for future studies aimed at overcoming the emerging problem of MPs/NPs phytotoxicity in edible crops.

Efficacy of zinc-based nanoparticles in alleviating the abiotic stress in plants: current knowledge and future perspectives.

Due to sessile, plants are unable to avoid unfavorable environmental conditions which leads to inducing serious negative effects on plant growth, crop yield, and food safety. Instead, various approaches were employed to mitigate the phytotoxicity of these emerging contaminants from the soil-plant system. However, recent studies based on the exogenous application of ZnO NPs approve of their important positive potential for alleviating abiotic stress-induced phytotoxicity leads to ensuring global food security. In this review, we have comprehensively discussed the promising role of ZnO NPs as alone or in synergistic interactions with other plant growth regulators (PGRs) in the mitigation of various abiotic stresses, i.e., heavy metals (HMs), drought, salinity, cold and high temperatures from different crops. ZnO NPs have stress-alleviating effects by regulating various functionalities by improving plant growth and development. ZnO NPs are reported to improve plant growth by stimulating diverse alterations at morphological, physiological, biochemical, and ultrastructural levels under abiotic stress factors. We have explained the recent advances and pointed out research gaps in studies conducted in earlier years with future recommendations. Thus, in this review, we have also addressed the opportunities and challenges together with aims to uplift future studies toward effective applications of ZnO NPs in stress management.

Seed priming with nano-silica effectively ameliorates chromium toxicity in Brassica napus.

Plant yield is severely hampered by chromium (Cr) toxicity, affirming the urgent need to develop strategies to suppress its phyto-accumulation. Silicon dioxide nanoparticles (SiO2 NPs) have emerged as a provider of sustainable crop production and resistance to abiotic stress. But, the mechanisms by which seed-primed SiO2 NPs palliate Cr-accumulation and its toxic impacts in Brassica napus L. tissues remains poorly understood. To address this gap, present study examined the protective efficacy of seed priming with SiO2 NPs (400 mg/L) in relieving the Cr (200 µM) phytotoxicity mainly in B. napus seedlings. Results delineated that SiO2 NPs significantly declined the accumulation of Cr (38.7/35.9%), MDA (25.9/29.1%), H2O2 (27.04/36.9%) and O2• (30.02/34.7%) contents in leaves/roots, enhanced the nutrients acquisition, leading to improved photosynthetic performance and better plant growth. SiO2 NPs boosted the plant immunity by upregulating the transcripts of antioxidant (SOD, CAT, APX, GR) or defense-related genes (PAL, CAD, PPO, PAO and MT-1), GSH (assists Cr-vacuolar sequestration), and modifying the subcellular distribution (enhances Cr-proportion in cell wall), thereby confer tolerance to ultrastructural damages under Cr stress. Our first evidence to establish the Cr-detoxification by seed-primed SiO2 NPs in B. napus, indicated the potential of SiO2 NPs as stress-reducing agent for crops grown in Cr-contaminated areas.

OsRbohI Regulates Rice Growth and Development via Jasmonic Acid Signalling.

Reactive oxygen species (ROS) are highly reactive molecules, generated by nicotinamide adenine dinucleotide phosphate oxidases encoded by respiratory burst oxidase homologs. The functions of the OsRbohs gene family in rice are diverse and poorly understood. OsRbohI was recently identified as a newly evolved gene in the rice OsRbohs gene family. However, the function of OsRbohI in regulating rice growth is not yet reported. In this study, our results indicate that knockout (KO) OsRbohI mutants showed significantly shorter shoot and primary roots, along with lower ROS content than the control lines, whereas the overexpression (OE) lines displayed contrasting results. Further experiments showed that the abnormal length of the shoot and root is mainly caused by altered cell size. These results indicate that OsRbohI regulates rice shoot and root growth through the ROS signal. More importantly, RNA-seq analysis and jasmonic acid (JA) treatment demonstrated that OsRbohI regulates rice growth via the JA synthesis and signaling pathways. Compared with the control, the results showed that the KO mutants were more sensitive to JA, whereas the OE lines were less sensitive to JA. Collectively, our results reveal a novel pathway in which OsRbohI regulates rice growth and development by affecting their ROS homeostasis through JA synthesis and signaling pathway.

Melatonin-mediated resistance to copper oxide nanoparticles-induced toxicity by regulating the photosynthetic apparatus, cellular damages and antioxidant defense system in maize seedlings.

The pollution of nanoparticles (NPs) has linked with severe negative effects on crop productivity. Thus, effective strategies are needed to mitigate the phytotoxicity of NPs. The aim of present study was to evaluate the efficacy of exogenously applied melatonin (MT) in mitigating the toxic effects of copper oxide nanoparticles (CuO NPs) from maize seedlings. Therefore, we comprehensively investigated the inhibitory effects of MT against CuO NPs-induced toxicity on morpho-physiological, biochemical and ultrastructural levels in maize. Our results show that CuO NPs (300 mg L-1) exposure displayed significantly reduction in all plant growth traits and induced toxicity in maize. Furthermore, 50 µM MT provided maximum plant tolerance against CuO NPs-induced phytotoxicity. It was noticed that MT improved plant growth, biomass, photochemical efficiency (Fv/Fm), chlorophyll contents (Chl a and Chl b), SPAD values and gas exchange attributes (stomatal conductance, net photosynthetic rate, intercellular CO2 concentration and transpiration rate) under CuO NPs stress. In addition, MT enhanced the antioxidant defense system and conferred protection to ultrastructural (mainly chloroplast, thylakoids membrane and plastoglobuli) damages and stomatal closure in maize plants subjected to CuO NPs stress. Together, it can be stated that the exogenous supply of MT improves the resilience of maize plants against the CuO NPs-induced phytotoxicity. Our current findings can be useful for the enhancement of plant growth and yield attributes in CuO NPs-contaminated soils. The reported information can provide insight into the MT pathways that can be used to improve crop stress tolerance in a challenging environment.

Ethylene accelerates copper oxide nanoparticle-induced toxicity at physiological, biochemical, and ultrastructural levels in rice seedlings.

The enormous use of metal-based nanoparticles (NPs) in different sectors may result in enhanced accumulation in agricultural soil, which could impose negative effects on crop productivity. Hence, strategies are needed to explore the mechanisms of copper oxide nanoparticle (CuO NP)-induced toxicity in crops. The present study aimed to investigate the involvement of ethylene in CuO NP-induced toxicity in rice seedlings. Here, our results indicate that 450 mg L-1 of CuO NPs induced toxic effects in rice seedlings. Thus, it was evidenced by the reduced plant biomass accumulation, enhanced oxidative stress indicators, and cellular ultrastructural damages. More importantly, the exogenous supply of ethylene biosynthesis and signaling antagonists cobalt (Co) and silver (Ag) respectively provided tolerance and improved the defense system of rice seedlings against CuO NP toxicity. The ethylene antagonists could significantly reduce the extent of ultrastructural and stomatal damage by controlling the ROS accumulation in rice seedlings under CuO NP stress. Furthermore, Co and Ag augmented the antioxidant defense system against CuO NP-induced toxicity. Contrary to that, all oxidative damage attributes were further enhanced exogenous application of ethylene biosynthesis precursor [1-aminocyclopropane-1-carboxylic acid (ACC)] in the presence of CuO NPs. In addition, ACC could increase the CuO NP-induced stomatal and ultrastructural damages by reducing the ROS-scavenging ability in rice seedlings. Taken together, these results indicate the involvement of ethylene in CuO NP-induced toxicity in rice seedlings.

Seed priming with zinc oxide nanoparticles downplayed ultrastructural damage and improved photosynthetic apparatus in maize under cobalt stress.

It is widely known that cobalt (Co) stress adversely affects plant growth and biomass accumulation, posing serious threats to crop production and food security. Nanotechnology is an emerging field in crop sciences for its potential in improving crop production and mitigating various stresses. Although there have been several studies reporting the toxic effects of zinc oxide nanoparticles (ZnO NPs) on different crops, their role in ameliorating heavy metal toxicity are still poorly understood. This study aimed to investigate the beneficial effects of seed priming with ZnO NPs in mitigating the phytotoxicity induced by Co stress. Our results demonstrated that ZnO NPs significantly improved the plant growth, biomass, and photosynthetic machinery in maize under Co stress. The NPs priming reduced ROS and MDA accumulations in maize shoots. More importantly, ZnO NPs alleviated the toxic effects of Co by decreasing its uptake and conferred stability to plant ultra-cellular structures and photosynthetic apparatus. Furthermore, a higher accumulation of nutrient content and antioxidant enzymes were found in NPs-primed seedlings. Collectively, we provide first evidence to demonstrate the alleviation of Co toxicity via ZnO NPs seed priming in maize, thus, illustrating the potential role of ZnO NPs to be applied as a stress mitigation agent for the crops grown in Co contaminated areas to enhance crop growth and yield.

Ethylene participates in zinc oxide nanoparticles induced biochemical, molecular and ultrastructural changes in rice seedlings.

Nowadays, the applications of engineered nanoparticles (ENPs) have been significantly increased, thereby negatively affecting crop production and ultimately contaminating the food chain worldwide. Zinc oxide nanoparticles (ZnO NPs) induced oxidative stress has been clarified in previous studies. But until now, it has not been investigated that how ethylene mediates or participates in ZnO NPs-induced toxicity and related cellular ultrastructural changes in rice seedlings. Here, we reported that 500 mg/L of ZnO NPs reduced the fresh weight (54.75% and 55.64%) and dry weight (40.33% and 47.83%) in shoot and root respectively as compared to control. Furthermore, ZnO NPs (500 mg/L) reduced chlorophyll content (72% Chla, 70% Chlb), induced the stomatal closure and ultrastructural damages by causing oxidative stress in rice seedlings. These cellular damages were significantly increased by exogenous applications of ethylene biosynthesis precursor (ACC) in the presence of ZnO NPs. In contrary, ZnO NPs induced damages on the above-mentioned attributes were reversed through the exogenous supply of ethylene signaling and biosynthesis antagonists such as silver (Ag) and cobalt (Co) respectively. Interestingly, ZnO NPs accelerate ethylene biosynthesis by up-regulating the transcriptome of ethylene biosynthesis responsive genes. The antioxidant enzymes activities and related gene expressions were further increased in ethylene signaling and biosynthesis associated antagonists (Ag and Co) treated seedlings as compared to sole ZnO NPs treatments. In contrary, the above-reported attributes were further decreased by ACC together with ZnO NPs. In a nutshell, ethylene effectively contributes in ZnO NPs induced toxicity and causing ultrastructural and stomatal damage in rice seedlings. Such findings could have potential implications in producing genetic engineered crops, which will be able to tolerate nanoparticles toxicity in the environment.

Salicylic acid underpins silicon in ameliorating chromium toxicity in rice by modulating antioxidant defense, ion homeostasis and cellular ultrastructure.

Chromium (Cr) phytotoxicity affirmed the need of mitigation strategies to remediate polluted soils and restricts its accumulation in the food chains. Salicylic acid (SA) and silicon (Si) play pivotal roles in stimulating the plant performance and stress resilience. So far, their interactive effects against Cr-phytotoxicities are less known. Thus, we evaluated the beneficial roles of alone or/and combine applications of SA and Si in mitigating the toxic effects of Cr in the leaves and roots of rice (Oryza sativa) seedlings. Results indicated that SA (10 µM) and/or Si (5 µM) markedly retrieved the Cr (100 µM) induced toxicities by minimizing the Cr-accretion in both leaves and roots, enhancing the performance of light harvesting pigments (total chlorophylls and carotenoids), water retention and accumulation of osmolytes (water-soluble protein and total soluble sugars) and ultimately improved the growth and biomass. Additionally, SA and/or Si maintained the ionic balance by enhancing the nutrients transport, upregulated the ascorbate-glutathione (AsA-GSH) cycle enzymes, minimized the extra accumulation of reactive oxygen species (ROS) (H2O2 and O2•‒), malondialdehyde (MDA), recovered the membrane stability and damages in cellular ultrastructure in Cr-stressed rice plants. Overall findings suggested that SA underpins Si in mitigating the Cr-induced phytotoxicities on the above-reported parameters and combined applications of SA and Si were more effective than alone treatments. The uptake or cellular accumulation of Cr, osmoprotectants level and antioxidant defense system against oxidative stress can be considered as key toxicity biomarkers for the safe cultivation of rice in Cr-contaminated soils.

BnaA02.NIP6;1a encodes a boron transporter required for plant development under boron deficiency in Brassica napus.

Boron (B) is an essential micronutrient for the plant normal growth. In Arabidopsis, NIP6;1 is a boric acid channel required for the proper distribution of boric acid, especially in the nodal regions of shoots. BnaA02.NIP6;1a, a homologous gene of AtNIP6;1 in Brassica napus, was reported to play a key role in B transport activity. However, little is known about the other functions of BnaA02.NIP6;1a in Brassica napus. In this study, we found that BnaA02.NIP6; 1a was localized in both plasma membrane and cytoplasm, which was different from that in Arabidopsis. The transgenic Arabidopsis plant containing a BnaA02.NIP6;1a promoter driven GUS reporter gene displayed strong GUS activity in roots, stems, leaves, especially in buds and open flowers, which are different from the expression pattern from its homologous gene in Arabidopsis. Silencing BnaA02.NIP6;1a repressed vegetative growth under B-deficient condition in Brassica napus. More importantly, knockdown of BnaA02.NIP6;1a in rapeseed resulted in the reduction of boron accumulation in the flower under boron deficiency and lead to severe sterility, which has not yet been reported before. Furthermore, nip6;1 mutant in Arabidopsis only showed the loss of apical dominance phenotype under boron deficiency at reproductive stage, whereas BnaA02.NIP6;1 RNAi lines exhibited large amounts of abnormal development of the inflorescence as compared with the wild type under boron limitation. Taken together, our results demonstrate that BnaA02.NIP6;1a encodes a boron transporter required for plant development under boron deficiency in Brassica napus, which shows its novel and diverse function in rapeseed compared with model plant Arabidopsis.

The WRKY6 transcription factor affects seed oil accumulation and alters fatty acid compositions in Arabidopsis thaliana.

In rapeseed, the oil content of the seed not only supplies energy for seed germination and seedling development but also provides essential dietary nutrients for humans and livestock. Recent studies have revealed that many transcription factors (TFs) regulate the accumulation of fatty acids (FAs) during seed development. WRKY6, a WRKY6 family TF, was reported to serve a function in the plant senescence processes, pathogen defense mechanisms and abiotic stress responses. However, the precise role of WRKY6 in influencing FA accumulation in seeds is still unknown. In this study, we demonstrate that WRKY6 has a high expression level in developing seeds and plays an essential role in regulating the accumulation of FAs in developing seeds of Arabidopsis. Mutation of WRKY6 resulted in significant increase in seed size, accompanied by an increase in FA content and changes in FA composition. Ultrastructure analyses showed that the absence of WRKY6 resulted in more and higher percentage of oil body in the cell of mature seeds. Quantitative real-time PCR analysis revealed changes in the expression of several genes related to photosynthesis and FA biosynthesis in wrky6 mutants at 10 or 16 days after pollination. These results reveal a novel function of WRKY6 influencing seed oil content and FAs compositions. This gene could be used as a promising gene resource to improve FA accumulation and seed yield in Brassica napus through genetic manipulation.